Large Scale Targeted Protein Quantification Using HR/AM Selected Ion Monitoring with MS/MS Confirmation on a Novel Hybrid, Q-OT-qIT Mass Spectrometer
Authors: Reiko Kiyonami1, Michael Senko1, Vlad Zabrouskov1, Jarrett Egertson2, Sonia Ting2, Michael MacCoss2, Andreas FR Hühmer1, José Felipe Lugão3
1Thermo Fisher Scientific, San Jose, CA, USA; 2University of Washington, Seattle, WA, USA; 3Nova Analítica, São Paulo, SP, BR
Resumo do Pôster:
Proteomics studies are turning from qualitative to quantitative to understand the biological function and interactions of proteins in biological systems. The extreme complexity and dynamic range of proteins in typical samples challenges traditional data dependent workflows by requiring very fast MS/MS acquisition to reproducibly and deeply interrogate the sample. Recently, several data independent acquisition approaches1 have been explored to increase reproducibility and comprehensiveness for better quantification. These approaches use targeted extracted fragment ions from HR/AM MS/MS data collected with a wide isolation window such as 25 Da for quantification. The quantitative performance could be potentially compromised by interfering fragments from co-eluted background compounds2.